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ADInstruments ad-converted
Effect of NO on biophysical parameters of isolated zebrafish myocyte Ca 2+ transients. (A) Photomicrography of isolated zebrafish myocytes under light microscope. (B) Photomicrography of the same myocyte shown in (A) loaded with 10 μM Fluo4-AM and seen under <t>fluorescence</t> microscope. (C) Representative Ca 2+ transient's traces obtained from electrical field stimulation. Control represented in black, 00 μM SNAP in blue, and 5 mM L-NAME in red. Statistical analyses of Ca 2+ transients' biophysical parameters: (D) Peak (nM), (E) Duration 50 (ms), (F) Peak Area (nM * s), (G) Time to Peak (ms), (H) Slope (μM/s), (I) Uptake Time (ms), and (J) Tau (s). Values are expressed as median; 25–75%. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; n = 278 cells for control, 153 cells for SNAP and 106 cells for L-NAME.
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Hugo Sachs Elektronik ecg amplifier type 689
Effect of NO on biophysical parameters of isolated zebrafish myocyte Ca 2+ transients. (A) Photomicrography of isolated zebrafish myocytes under light microscope. (B) Photomicrography of the same myocyte shown in (A) loaded with 10 μM Fluo4-AM and seen under <t>fluorescence</t> microscope. (C) Representative Ca 2+ transient's traces obtained from electrical field stimulation. Control represented in black, 00 μM SNAP in blue, and 5 mM L-NAME in red. Statistical analyses of Ca 2+ transients' biophysical parameters: (D) Peak (nM), (E) Duration 50 (ms), (F) Peak Area (nM * s), (G) Time to Peak (ms), (H) Slope (μM/s), (I) Uptake Time (ms), and (J) Tau (s). Values are expressed as median; 25–75%. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; n = 278 cells for control, 153 cells for SNAP and 106 cells for L-NAME.
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Image Search Results


Effect of NO on biophysical parameters of isolated zebrafish myocyte Ca 2+ transients. (A) Photomicrography of isolated zebrafish myocytes under light microscope. (B) Photomicrography of the same myocyte shown in (A) loaded with 10 μM Fluo4-AM and seen under fluorescence microscope. (C) Representative Ca 2+ transient's traces obtained from electrical field stimulation. Control represented in black, 00 μM SNAP in blue, and 5 mM L-NAME in red. Statistical analyses of Ca 2+ transients' biophysical parameters: (D) Peak (nM), (E) Duration 50 (ms), (F) Peak Area (nM * s), (G) Time to Peak (ms), (H) Slope (μM/s), (I) Uptake Time (ms), and (J) Tau (s). Values are expressed as median; 25–75%. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; n = 278 cells for control, 153 cells for SNAP and 106 cells for L-NAME.

Journal: Frontiers in Physiology

Article Title: NO-sGC Pathway Modulates Ca 2+ Release and Muscle Contraction in Zebrafish Skeletal Muscle

doi: 10.3389/fphys.2017.00607

Figure Lengend Snippet: Effect of NO on biophysical parameters of isolated zebrafish myocyte Ca 2+ transients. (A) Photomicrography of isolated zebrafish myocytes under light microscope. (B) Photomicrography of the same myocyte shown in (A) loaded with 10 μM Fluo4-AM and seen under fluorescence microscope. (C) Representative Ca 2+ transient's traces obtained from electrical field stimulation. Control represented in black, 00 μM SNAP in blue, and 5 mM L-NAME in red. Statistical analyses of Ca 2+ transients' biophysical parameters: (D) Peak (nM), (E) Duration 50 (ms), (F) Peak Area (nM * s), (G) Time to Peak (ms), (H) Slope (μM/s), (I) Uptake Time (ms), and (J) Tau (s). Values are expressed as median; 25–75%. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; n = 278 cells for control, 153 cells for SNAP and 106 cells for L-NAME.

Article Snippet: Three consecutive stimulations at 0.5 Hz 18 V for 10 ms were given and the change in fluorescence intensity was AD-converted (Powerlab 15T, AD Instruments) and sampled at 10 KHz in a PC using LabChart Pro7 software (AD Instruments).

Techniques: Isolation, Light Microscopy, Fluorescence, Microscopy, Control